MPFREE
Science & Detection Methods

µFTIR, Raman, and Py-GC-MS: What Each Method Actually Measures

None of the three methods used in the MPFREE Standard measures the same thing. Here's the actual difference.

6 min read · Updated 2026-09

µFTIR: particle count and identity, down to about 20 µm

Micro-Fourier-transform infrared spectroscopy (µFTIR) shines infrared light through a particle and matches the absorption spectrum against a reference library to identify the polymer. Its conventional practical size floor is around 20 µm [1]; below that, particle-to-noise ratio degrades and confident identification gets difficult. Newer variants like large-area ATR-FTIR have pushed detection down to roughly 1.3 µm in research settings [2], but that isn't yet the routine floor most accredited labs run at scale.

Raman microspectroscopy: finer resolution, same basic approach

Raman microspectroscopy uses a different physical mechanism (inelastic light scattering rather than absorption) but produces a similar output: particle count plus polymer identity via spectral matching. Its finer laser spot size gets it down to about 1 µm in practice [3] — an order of magnitude below conventional µFTIR. The tradeoff is analysis speed and, historically, the cost of comprehensive reference libraries, though open-access libraries have started closing that gap [4].

Py-GC-MS: total mass, no size or shape at all

Pyrolysis-gas chromatography–mass spectrometry (Py-GC-MS) works completely differently: it thermally decomposes the entire sample and identifies polymers from their breakdown products. It reports mass, not particle count — commonly down to nanogram levels across a dozen common polymers [5] — and because it destroys the sample, it produces no data on particle size, shape, or count [6].

That's precisely why it matters for the sub-1-µm range neither optical method can resolve: Py-GC-MS doesn't have a size floor at all, because it isn't sizing anything — it's measuring total polymer mass, including whatever nanoplastic fraction is present. A standard built on only the two optical methods would have a structural blind spot below about 1 µm; that's the specific gap this third method is there to close.

Sources

  1. [1] ACS Omega (2022). "Detection of Sub-20 μm Microplastic Particles by Attenuated Total Reflection Fourier Transform Infrared Spectroscopy and Comparison with Raman Spectroscopy." View source →
  2. [2] Marine Pollution Bulletin (2023). "Detecting small microplastics down to 1.3 μm using large area ATR-FTIR." View source →
  3. [3] PMC (2023). "TUM-ParticleTyper 2: automated quantitative analysis of (microplastic) particles and fibers down to 1 μm by Raman microspectroscopy." View source →
  4. [4] Scientific Data / Nature (2022). "A Raman spectral reference library of potential anthropogenic and biological ocean polymers." View source →
  5. [5] PMC (2024). "Rapid and Sensitive Quantification of Nano- and Microplastics in Water, Sediment, and Biological Tissue by Pyrolysis-Gas Chromatography Tandem Mass Spectrometry with Dynamic Reaction Monitoring." View source →
  6. [6] PMC (2023). "Analysis of microplastics in the environment: Identification and quantification of trace levels of common types of plastic polymers using pyrolysis-GC/MS." View source →